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Neuronal Cell Culture pp 195–201Cite as

Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2311))

Abstract

The lack of a convenient in vitro human neuronal model to study alcohol-induced neurodegenerative diseases, such as fetal alcohol syndrome (FAS), prompted us to develop human neuronal culture and in vitro human FAS model by incubating cells with physiologically relevant EtOH concentration (50 mM). Here, we describe the detailed method of isolation of human neuronal culture, and ability to analyze neurites extension using Sholl assay. We utilized highly efficient transfection method of neuronal cells to study morphology of neurons with or without EtOH treatment.

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Author information

Authors and Affiliations

  1. Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA

    Nune Darbinian & Kamel Khalili

  2. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

    Armine Darbinyan

  3. Department of Biology, College of Science & Technology, Temple University, Philadelphia, PA, USA

    Shohreh Amini

  4. Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine, Temple University , Philadelphia, PA, USA

    Nune Darbinian

Authors
  1. Nune Darbinian
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  2. Armine Darbinyan
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  3. Kamel Khalili
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  4. Shohreh Amini
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Corresponding author

Correspondence to Nune Darbinian .

Editor information

Editors and Affiliations

  1. College of Science and Technology, Temple University, Philadelphia, PA, USA

    Shohreh Amini

  2. Department of Neuroscience, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA

    Martyn K. White

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Darbinian, N., Darbinyan, A., Khalili, K., Amini, S. (2021). Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay. In: Amini, S., White, M.K. (eds) Neuronal Cell Culture. Methods in Molecular Biology, vol 2311. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1437-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1437-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1436-5

  • Online ISBN: 978-1-0716-1437-2

  • eBook Packages: Springer Protocols

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